Year: 2015 | Month: August | Volume 5 | Issue 2
In Vitro Maturation of Bubaline Oocytes in Three Different Culture Media (TCM 199, Ham’s F 10 and Way mouth MB medium)
Abstract:
In order to evaluate the effect of media on in vitro maturation, culturable grade buffalo oocytes were matured in vitro in three different media (Ham’s F-10, Waymouth MB and TCM-199) with same supplements (5µg/ml FSH, 5µg/ml LH, 1µg/ml estradiol, 25 mM Hepes, 0.25 mM pyruvate and antibiotics). The overall mean culturable grade oocyte recovery was 3.12±0.20. At the end of experiment all oocytes in all groups were fixed and stained to evaluate the nuclear status. Oocytes were considered mature if they were at metaphase II. Significantly higher (P<0.01) proportion of oocytes were matured in vitro in Waymouth medium compared to Ham’s F-10. In TCM-199, IVM rates were non-significantly higher compared to Ham’s F-10 and non-significantly lower compared to Waymouth MB medium. It was concluded that Way mouth MB medium is the most appropriate medium for in vitro maturation of buffalo oocytes followed by TCM-199 and Ham’s F 10.
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